Inorganic Phosphate and Enhanced Glucose Degradation by the Intact Erythrocyte.

INHIBITION OF HUMAN ERYTHROCYTE GLUCOSE 6-PHOSPHATE DEHYDROGENASE ACTIVITY BY DEHYDROEPIANDROSTERONE AND RELATED STEROIDS.

The inhibitory effects of several steroids on G6PD activity using intact erythrocytes are reported. Incubation of whole blood with dehydroepiandrosterone (DHEA) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. Addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a po...

Inhibition of Human Erythrocyte Glucose 6-Phosphate Dehydrogenase by Cadmium, Nickel and Aluminium

Characterization of starch breakdown in the intact spinach chloroplast.

Starch degradation with a rate of 1 to 2 microgram-atom carbon per milligram chlorophyll per hour was monitored in the isolated intact spinach (Spinacia oleracea) chloroplast which had been preloaded with (14)C-starch photosynthetically from (14)CO(2). Starch breakdown was dependent upon inorganic phosphate and the (14)C-labeled intermediates formed were principally those of the Embden-Meyerhof...

THE FORMATION OF GLUCOSE-l-PHOSPHORIC ACID IN EXTRACTS OF MAMMALIAN TISSUES AND OF YEAST

The initial stages of fermentation of glycogen to lactic acid in muscle have been investigated only recently. It was shown (1, 2) that the glycolytic reactions are initiated by the formation of glucose-l-phosphoric acid from glycogen and inorganic phosphate, an enzymatic process in which adenylic acid acts as coenzyme. The glucose-l-phosphoric acid (l-ester) is converted to glucose-6phosphoric ...

Optimizing the production of Polyphosphate from Acinetobacter towneri

Inorganic polyphosphates (PolyP) are linear polymers of few to several hundred orthophosphate residues, linked by energy-rich phosphoanhydride bonds. Four isolates had been screened from soil sample. By MALDI-TOF analysis, they were identified as Bacillius cereus, Acinetobacter towneri, B. megaterium and B. cereus. The production of PolyP in four isolates was studied in phosph...